In a shocking twist, Callie – of Squid Sisters fame – has gone missing and her right-tentacle squid Marie tasks the player to search for Callie and the Great Zapfish. Players can use different weapons in single-player mode to overcome obstacles, defeat enemies and take down towering bosses – weapons like the Roller, Slosher, Splatling or the new Dualies. The single-player mode is designed to teach players tips and tricks that they can then use in multiplayer.