Next, color the pupils. Lock transparent pixels and pick a light color for the pupil layer.

This time I used this color.

Lay a slightly darker color on top using the gradation tool.

The gradation settings should look like this.

mode: normal

Opacity

opposite direction

dither

transparent part

Add one more level of dark color. This time it's a more bluish color.

That was a bit too blue, so I adjusted the color in quality adjustment→color→hue/saturation. I've added 5 to the hue.

editing

master

hue

Use the syringe to take color from this dark area. Create a new layer on top called "pupil (dark)". Swirl the brush around in the middle with the color you've sampled.

I used the brush to lightly cover the color in these areas of "pupil" and "pupil (dark)".

Keep sampling color from the dark parts as you did in step 5, and apply the color by tracing the contour.

Now make a "pupil (light)" layer, make the layer overlay, and lightly trace this area. This time I used this color.

Using a dark color, put a small circle in the middle. Finally, create a new layer above the line layer, and add the highlights. This time, un-check "opacity". Erase the part that covers the hair.

pupil highlights

line art

fang

mouth

skin

pupil (light)

pupil (dark)

pupil

white